Scientific Expertise
Animal Expertise (Handling and Surgery): Efficient in animal handling (especially Wistar rats)
Animal tissue culture techniques: Proficiency in Mammalian Tissue culture for various types of normal (L6 myotube and 3T3-L1 adipocyte) and cancerous cell lines (HEK293, Hep G2 and HeLa) cell lines
Oxidative Stress Study: Proficiency in biochemical estimation of enzymatic (SGOT/SGPT, ALP, GGT, Catalase, SOD, GST, and GSH) and antioxidant assays
Molecular Biology Techniques: Isolation of genomic DNA and RNA, PCR, Purification of plasmid DNA by CsCl density gradient method, gel electrophoresis and RT-PCR
Microbiological Techniques: Experience on bacterial culture, pure culture isolation, and antibiotic susceptibility test
Biochemical Techniques: Quantitative and qualitative analysis of biomolecules and enzyme assay
Analytical Techniques: (a) New enzymatic method developed for the glucose uptake in the cell lines (b) Spectroscopic techniques for analyses – UV-Vis, fluorescence, FTIR (c) Hands-on experience in handling HPLC, GPC, FTIR, Gel Dock, Elisa reader
Relevant Skills : (a) Expert Level: Windows operating systems and application software: MS Office, ChemDraw, Hyperchem, Reference Management Software (Endnote Plus), Prism Graph Pad, Sigma Stat (Jandel Scientific), Sigma Plot, Origin, SPSS, Adobe Photoshop. (b) Expert Level: Scientific data retrieval from various Internet portals like Sciencedirect, Pubmed, Highwire, and Scopus, etc. (c) Beginner Level: Insight2, Matlab